|
KMID : 0604520110370040309
|
|
Journal of the Society of Cosmetic Scientists of Korea
2011 Volume.37 No. 4 p.309 ~ p.318
|
|
|
Antioxidative Activity and Component Analysis of Phellinus linteus Extracts
|
|
Kim A-Reum
Kim Jung-Eun
Park Soo-Nam
|
|
|
Abstract
|
|
|
|
In this study, the antioxidative effect, antibacterial, inhibitory effects on tyrosinase, inhibitory effects on elastase and component analysis of Phellinus linteus (P. linteus) extracts were investigated. The ethyl acetate fraction of P. linteus extracts (2.94 μg/mL) showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50). Reactive oxygen species (ROS) scavenging activity (OSC50) of P. linteus extracts on ROS generated in Fe3+-EDTA/H2O2 system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction (0.0072 μg/mL) showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of P. linteus extracts on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The P. linteus extracts showed cellular membrane protective effects in a concentration dependent manner (5 ~ 50 μg/mL). The inhibitory effect (IC50) on tyrosinase of P. linteus extract was the highest at 50 % ethanol extract (6.34 μg/mL), and the inhibitory effect (IC50) on elastase of P. linteus was the highest at ethyl acetate fraction (14.08 μg/mL). TLC, HPLC chromatogram and LC/ESI-MS of the ethyl acetate fraction obtained from P. linteus extracts were identified interfungin A (PL RPT-1a). These results indicate that extract/fractions of P. linteus can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging ROS, and protect cellular membranes against ROS. Extract/fractions of P. linteus can be applicable to new cosmeceuticals for antioxidant, antiaging, antiwrinkle and whitening.
|
|
|
KEYWORD
|
|
|
Phellinus linteus, antioxidative activity, antiaging activity, LC/ESI-MS, component analysis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|